Single-layer exfoliated reduced graphene oxide-antibody Tau sensor for detection in human serum

Author
Ye, Mengsha
Jiang. Mingdi
Cheng, Jing
Li, Xuanying
Liu, Ziwei
Zhang, Weiying
Mugo, Samuel
Jaffrezic-Renault, Nicole
Guo, Zhenzhong
Faculty Advisor
Date
2020
Keywords
Alzheimer's disease , Tau-441 , electrochemical antibody sensor , single layer reduced graphene oxide (rGO) , 1,3,6,8-pyrenetetrasulfonic acid tetrasodium salt , (PTSA)
Abstract (summary)
This study presents a novel electrochemical sensor for detection of Tau-441, an Alzheimer's disease (AD) biomarker protein. The sensor comprised a glassy carbon electrode modified by chemically exfoliated single-layer reduced graphene oxide (rGO) grafted with anti-Tau antibody. Using square wave voltammetry (SWV), the electrochemical sensor yielded a linear response to Tau-441 in the 0.08 pM-80 pM concentration range, with a low detection limit of 75 fM. The sensor has been successfully tested in the detection of Tau-441 of human serum from participants with and without AD, therefore it is shown to be an efficient and effective tool for the early diagnosis of AD.
Publication Information
M. Ye, M. Jiang, J. Cheng, X. Li; Z. Liu, W. Zhang, S.M. Mugo, N. Jaffrezic-Renault ; Z. Guo. Single-layer exfoliated reduced graphene oxide-antibody Tau sensor for detection in human serum. Sensors and Actuators B: Chemical. 2020, 308, 127692. DOI: 10.1016/j.snb.2020.127692
DOI
Notes
Item Type
Article
Language
English
Rights
All Rights Reserved