Repository logo
 

BldG and SCO3548 interact antagonistically to control key developmental processes in streptomyces coelicolor

Faculty Advisor

Date

2009

Keywords

proteins, streptomyces coelicolor, genetics

Abstract (summary)

The similarity of BldG and the downstream coexpressed protein SCO3548 to anti-anti-sigma and anti-sigma factors, respectively, together with the phenotype of a bldG mutant, suggests that BldG and SCO3548 interact as part of a regulatory system to control both antibiotic production and morphological differentiation in Streptomyces coelicolor. A combination of bacterial two-hybrid, affinity purification, and far-Western analyses demonstrated that there was self-interaction of both BldG and SCO3548, as well as a direct interaction between the two proteins. Furthermore, a genetic complementation experiment demonstrated that SCO3548 antagonizes the function of BldG, similar to other anti-anti-sigma/anti-sigma factor pairs. It is therefore proposed that BldG and SCO3548 form a partner-switching pair that regulates the function of one or more sigma factors in S. coelicolor. The conservation of bldG and sco3548 in other streptomycetes demonstrates that this system is likely a key regulatory switch controlling developmental processes throughout the genus Streptomyces.

Publication Information

Parashar, A., Colvin, K. R., Bignell, D. R. D., & Leskiw, B. K. (2009). BldG and SCO3548 interact antagonistically to control key developmental processes in Streptomyces coelicolor. Journal of Bacteriology, 191(8), 2541-2550. doi: 10.1128/JB.01695-08

DOI

Notes

Item Type

Article

Language

English

Rights

All Rights Reserved