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Circular permutated red fluorescent proteins and calcium ion indicators based on mCherry

Faculty Advisor

Date

2013

Keywords

Abstract (summary)

Red fluorescent indicators for calcium ion (Ca) are preferable, relative to blue-shifted alternatives, for biological imaging applications due to the lower phototoxicity, lower autofluorescent background and deeper tissue penetration associated with longer wavelength light. Accordingly, we undertook the development of a genetically encoded Ca indicator based on the popular and widely utilized Discosoma-derived red fluorescent protein, mCherry.

Publication Information

Carlson, H. J., & Campbell, R. E. (2013). Circular permutated red fluorescent proteins and calcium ion indicators based on mCherry. Protein Engineering, Design & Selection, 26(12), 763–772. https://doi.org/10.1093/protein/gzt052

Notes

Item Type

Article

Language

English

Rights

All Rights Reserved