Parashar, ArchanaBignell, Dawn R. D.Leskiw, Brenda K.Harcombe, Kimberley2018-01-192022-05-312022-05-312009Parashar, A., Colvin, K. R., Bignell, D. R. D., & Leskiw, B. K. (2009). BldG and SCO3548 interact antagonistically to control key developmental processes in Streptomyces coelicolor. Journal of Bacteriology, 191(8), 2541-2550. doi: 10.1128/JB.01695-08https://hdl.handle.net/20.500.14078/1130The similarity of BldG and the downstream coexpressed protein SCO3548 to anti-anti-sigma and anti-sigma factors, respectively, together with the phenotype of a bldG mutant, suggests that BldG and SCO3548 interact as part of a regulatory system to control both antibiotic production and morphological differentiation in Streptomyces coelicolor. A combination of bacterial two-hybrid, affinity purification, and far-Western analyses demonstrated that there was self-interaction of both BldG and SCO3548, as well as a direct interaction between the two proteins. Furthermore, a genetic complementation experiment demonstrated that SCO3548 antagonizes the function of BldG, similar to other anti-anti-sigma/anti-sigma factor pairs. It is therefore proposed that BldG and SCO3548 form a partner-switching pair that regulates the function of one or more sigma factors in S. coelicolor. The conservation of bldG and sco3548 in other streptomycetes demonstrates that this system is likely a key regulatory switch controlling developmental processes throughout the genus Streptomyces.enAll Rights Reservedproteinsstreptomyces coelicolorgeneticsArticle