Mugo, SamuelAlberkant, JonathanBernstein, NinaZenkina, Olena V.2022-07-252022-10-122022-10-122021Mugo, S. M., Alberkant, J., Bernstein, N., & Zenkina, O. V. (2021). Flexible electrochemical aptasensor for cortisol detection in human sweat. Analytical Methods 13, 4169-4173. https://doi.org/10.1039/d1ay01233ahttps://hdl.handle.net/20.500.14078/2776This communication demonstrates an electrochemical DNA aptasensor for the detection of cortisol in human sweat. The aptasensor was fabricated via layer-by-layer assembly on stretchable polydimethylsiloxane (PDMS) coated with conductive nanoporous carbon nanotube-cellulose nanocrystals (CNC/CNT) film using a linker to a cortisol specific DNA aptamer. The flexible cortisol aptasensor had a dynamic range of 2.5–35 ng mL−1. The aptasensor precision was determined to be 2.7% relative standard deviation (%RSD) across the concentration dynamic range. The aptasensor was determined to have a limit of detection (LOD) of ∼ 1.8 ng mL−1. The aptasensor was demonstrated to have high selectivity to cortisol and was unresponsive to interfering species including glucose, sodium lactate, and β-estradiol. The aptasensor was successfully evaluated for the detection of cortisol in human sweat indicative of its high specificity.enAll Rights ReservedaptasensorscortisolArticlehttps://doi.org/10.1039/d1ay01233a