Mugo, SamuelLu, Weihao2022-09-302022-10-122022-10-122021S.M. Mugo, W. Lu. Determination of β-Estradiol by Surface-Enhance Raman Spectroscopy (SERS) Using a Surface Imprinted Methacrylate Polymer on Nanoporous Biogenic Silica. Analytical Letters, 2021. https://doi.org/10.1080/00032719.2021.1932969https://hdl.handle.net/20.500.14078/2826A phytoplankton derived nanoporous biogenic silica (BS) scaffold grafted with polymethacrylate β-estradiol molecularly imprinted polymers (MIP) film (MIP@BS) is reported as a selective platform for the determination of β-estradiol using surfaced-enhanced Raman scattering (SERS). The biogenic silica nanoparticles provided high surface area support for the MIP while serving as an active signal enhancement substrate for SERS. The MIP@BS platform demonstrated an excellent linear response (R2 = 0.9996) between 0.1 and 4 ng/mL of β-estradiol standards. The limit of detection (LOD) for the MIP@BS SERS sensor for β-estradiol was 0.073 ng/mL. The MIP@BS SERS sensors were successfully evaluated for the determination of β-estradiol in milk, while demonstrating good stability and reusability.enAll Rights Reservedβ-estradiolβ-estradiol molecularly imprinted methacrylate polymersnanoporous biogenic silica (BS)surface enhanced Raman spectroscopy (SERS)surface polymerizationArticlehttps://doi.org/10.1080/00032719.2021.1932969