Browsing by Author "Zenkina, Olena V."
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Item Flexible electrochemical aptasensor for cortisol detection in human sweat(2021) Mugo, Samuel; Alberkant, Jonathan; Bernstein, Nina; Zenkina, Olena V.This communication demonstrates an electrochemical DNA aptasensor for the detection of cortisol in human sweat. The aptasensor was fabricated via layer-by-layer assembly on stretchable polydimethylsiloxane (PDMS) coated with conductive nanoporous carbon nanotube-cellulose nanocrystals (CNC/CNT) film using a linker to a cortisol specific DNA aptamer. The flexible cortisol aptasensor had a dynamic range of 2.5–35 ng mL−1. The aptasensor precision was determined to be 2.7% relative standard deviation (%RSD) across the concentration dynamic range. The aptasensor was determined to have a limit of detection (LOD) of ∼ 1.8 ng mL−1. The aptasensor was demonstrated to have high selectivity to cortisol and was unresponsive to interfering species including glucose, sodium lactate, and β-estradiol. The aptasensor was successfully evaluated for the detection of cortisol in human sweat indicative of its high specificity.