Flexible electrochemical aptasensor for cortisol detection in human sweat
Faculty Advisor
Date
2021
Keywords
aptasensors, cortisol
Abstract (summary)
This communication demonstrates an electrochemical DNA aptasensor for the detection of cortisol in human sweat. The aptasensor was fabricated via layer-by-layer assembly on stretchable polydimethylsiloxane (PDMS) coated with conductive nanoporous carbon nanotube-cellulose nanocrystals (CNC/CNT) film using a linker to a cortisol specific DNA aptamer. The flexible cortisol aptasensor had a dynamic range of 2.5–35 ng mL−1. The aptasensor precision was determined to be 2.7% relative standard deviation (%RSD) across the concentration dynamic range. The aptasensor was determined to have a limit of detection (LOD) of ∼ 1.8 ng mL−1. The aptasensor was demonstrated to have high selectivity to cortisol and was unresponsive to interfering species including glucose, sodium lactate, and β-estradiol. The aptasensor was successfully evaluated for the detection of cortisol in human sweat indicative of its high specificity.
Publication Information
Mugo, S. M., Alberkant, J., Bernstein, N., & Zenkina, O. V. (2021). Flexible electrochemical aptasensor for cortisol detection in human sweat. Analytical Methods 13, 4169-4173. https://doi.org/10.1039/d1ay01233a
Notes
Item Type
Article
Language
English
Rights
All Rights Reserved
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