Creation of controls for the identification of the nuclear localization sequence of FLOWERING LOCUS C in Arabidopsis thaliana
Author
Faculty Advisor
Date
2024
Keywords
nuclear localization sequence, nuclear localization mechanisms
Abstract (summary)
This project aims to create two controls for use in later honours projects identifying the nuclear localization sequence (NLS) of the MADS transcription factor FLOWERING LOCUS C (FLC) in Arabidopsis thaliana. First, I aimed to create a line of Arabidopsis expressing GFP using Agrobacterium GV3101 via the floral dip method. This will be used to show GFP fluorescence that is not specifically localized to the nucleus. Second, I worked on the removal of an internal BsaI site from FLC using site-directed mutagenesis to create an FLC:GFP fusion plasmid. This plasmid will be used to show that FLC localizes to the nucleus. Active nuclear import is required for large proteins, like MADS transcription factors, to translocate, which is needed for these proteins to perform their function in the correct location. Import is facilitated by the interaction of an importin and NLS, a specific amino acid sequence, of the cargo protein. Previously, research has characterized NLSs in other proteins as well as other domains of FLC. However, the NLS of FLC has not yet been identified. By contributing to the characterization of FLC’s NLS, I have contributed to the understanding of nuclear localization mechanisms. In extension, the continuation of this work will contribute to the knowledge of protein dimerization, transcriptional regulation, and plant development.
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DOI
Notes
Presented on April 19, 2024 at Student Research Day held at MacEwan University in Edmonton, Alberta.
Item Type
Student Presentation
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Embargoed Until:
2026-04-19