BldG and SCO3548 interact antagonistically to control key developmental processes in streptomyces coelicolor
Faculty Advisor
Date
2009
Keywords
proteins, streptomyces coelicolor, genetics
Abstract (summary)
The similarity of BldG and the downstream coexpressed protein SCO3548 to anti-anti-sigma and anti-sigma factors, respectively, together with the phenotype of a bldG mutant, suggests that BldG and SCO3548 interact as part of a regulatory system to control both antibiotic production and morphological differentiation in Streptomyces coelicolor. A combination of bacterial two-hybrid, affinity purification, and far-Western analyses demonstrated that there was self-interaction of both BldG and SCO3548, as well as a direct interaction between the two proteins. Furthermore, a genetic complementation experiment demonstrated that SCO3548 antagonizes the function of BldG, similar to other anti-anti-sigma/anti-sigma factor pairs. It is therefore proposed that BldG and SCO3548 form a partner-switching pair that regulates the function of one or more sigma factors in S. coelicolor. The conservation of bldG and sco3548 in other streptomycetes demonstrates that this system is likely a key regulatory switch controlling developmental processes throughout the genus Streptomyces.
Publication Information
Parashar, A., Colvin, K. R., Bignell, D. R. D., & Leskiw, B. K. (2009). BldG and SCO3548 interact antagonistically to control key developmental processes in Streptomyces coelicolor. Journal of Bacteriology, 191(8), 2541-2550. doi: 10.1128/JB.01695-08
DOI
Notes
Item Type
Article
Language
English
Rights
All Rights Reserved
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