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BldG and SCO3548 interact antagonistically to control key developmental processes in streptomyces coelicolor

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dc.contributor.authorParashar, Archana
dc.contributor.authorBignell, Dawn R. D.
dc.contributor.authorLeskiw, Brenda K.
dc.contributor.authorHarcombe, Kimberley
dc.date.accessioned2018-01-19
dc.date.accessioned2022-05-31T00:00:40Z
dc.date.available2022-05-31T00:00:40Z
dc.date.issued2009
dc.description.abstractThe similarity of BldG and the downstream coexpressed protein SCO3548 to anti-anti-sigma and anti-sigma factors, respectively, together with the phenotype of a bldG mutant, suggests that BldG and SCO3548 interact as part of a regulatory system to control both antibiotic production and morphological differentiation in Streptomyces coelicolor. A combination of bacterial two-hybrid, affinity purification, and far-Western analyses demonstrated that there was self-interaction of both BldG and SCO3548, as well as a direct interaction between the two proteins. Furthermore, a genetic complementation experiment demonstrated that SCO3548 antagonizes the function of BldG, similar to other anti-anti-sigma/anti-sigma factor pairs. It is therefore proposed that BldG and SCO3548 form a partner-switching pair that regulates the function of one or more sigma factors in S. coelicolor. The conservation of bldG and sco3548 in other streptomycetes demonstrates that this system is likely a key regulatory switch controlling developmental processes throughout the genus Streptomyces.
dc.description.urihttps://library.macewan.ca/cgi-bin/SFX/url.pl/9JW
dc.identifier.citationParashar, A., Colvin, K. R., Bignell, D. R. D., & Leskiw, B. K. (2009). BldG and SCO3548 interact antagonistically to control key developmental processes in Streptomyces coelicolor. Journal of Bacteriology, 191(8), 2541-2550. doi: 10.1128/JB.01695-08
dc.identifier.urihttps://hdl.handle.net/20.500.14078/1130
dc.languageEnglish
dc.language.isoen
dc.rightsAll Rights Reserved
dc.subjectproteins
dc.subjectstreptomyces coelicolor
dc.subjectgenetics
dc.titleBldG and SCO3548 interact antagonistically to control key developmental processes in streptomyces coelicoloren
dc.typeArticle
dspace.entity.type

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