Synthesis and characterization of mesoporous polymer-silica hybrid monolith using conventional sol-gel method for enzyme support
hybrid monolith, fused silica capillary, sol-gel polymerization, enzyme support, mesoporous monolith
This research involved developing a novel solid support for an enzyme attachment, focusing on synthesizing a polymer-silica hybrid monolith via in-situ sol-gel polymerization method. The fabrication of a very large surface area of the monolith was done using a cold mixture of poly(ethylene-glycol) (PEG) with tetraethyl-orthosilicate (TEOS) and acetic acid with different ratios of PEG amount and molecular weights, namely PEG-0.1, PEG-0.2, and PEG-0.3. The experiments were conducted at a very low temperature of 0 °C, followed by overnight gelification and aging. The sol then underwent calcination at 200 °C forming a hybrid monolith. The characterizations of hybrid monoliths were performed by Attenuated-Total Reflection–Fourier Transformed Infrared Spectroscopy (ATR-FTIR), Scanning Electron Microscope (SEM), and Surface Area and Porosity Analyzer using both Brunauer-Emmett-Teller (BET) and Barrett-Joyner-Halenda (BJH) methods to describe the developed monoliths. FTIR shows the presence of Si-O-Si stretching associated with the monolith network due to the polymerization process together with the presence of silanol functional group (Si-OH) that can be exploited further for covalent attachment with the enzyme. Results also showed that the optimum ratios for the hybrid polymer-silica synthesis were PEG-0.1with 10,000 Mn surface area of mesoporous network recorded for 494.121 m2/g and pore volume of 0.265 cm3/g. These findings showed that the synthesized hybrid monolith on fused silica capillary will provide a vast surface area with desirable functional groups; thus, very promising for lipase immobilization support that can be used in future small-scale lipid transformation.
Noor Hidayah Idrohani, S. M. Mugo, Sabiqah Tuan Anuar. Synthesis and characterization of mesoporous polymer-silica hybrid monolith using conventional sol-gel method for enzyme support. Malaysian Journal of Analytical Sciences, 22(1), 2018. DOI: 10.17576/mjas-2018-2201-03
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