Differential protein expression during growth on model and commercial mixtures of naphthenic acids in Pseudomonas fluorescens Pf-5
dc.contributor.author | McKew, Boyd A. | |
dc.contributor.author | Johnson, Richard | |
dc.contributor.author | Clothier, Lindsay | |
dc.contributor.author | Skeels, Karl | |
dc.contributor.author | Ross, Matthew S. | |
dc.contributor.author | Metodiev, Metodi | |
dc.contributor.author | Frenzel, Max | |
dc.contributor.author | Gieg, Lisa M. | |
dc.contributor.author | Martin, Jonathan W. | |
dc.contributor.author | Hough, Michael A. | |
dc.contributor.author | Whitby, Corinne | |
dc.date.accessioned | 2022-01-18 | |
dc.date.accessioned | 2022-05-31T01:45:10Z | |
dc.date.available | 2022-05-31T01:45:10Z | |
dc.date.issued | 2021 | |
dc.description.abstract | Naphthenic acids (NAs) are carboxylic acids with the formula (CnH2n+ZO2) and are among the most toxic, persistent constituents of oil sands process-affected waters (OSPW), produced during oil sands extraction. Currently, the proteins and mechanisms involved in NA biodegradation are unknown. Using LC-MS/ MS shotgun proteomics, we identified proteins overexpressed during the growth of Pseudomonas fluorescens Pf-5 on a model NA (4′-n-butylphenyl)- 4-butanoic acid (n-BPBA) and commercial NA mixture (Acros). By day 11, >95% of n-BPBA was degraded. With Acros, a 17% reduction in intensity occurred with 10–18 carbon compounds of the Z family −2 to −14 (major NA species in this mixture). A total of 554 proteins (n-BPBA) and 631 proteins (Acros) were overexpressed during growth on NAs, including several transporters (e.g., ABC transporters), suggesting a cellular protective response from NA toxicity. Several proteins associated with fatty acid, lipid, and amino acid metabolism were also overexpressed, including acyl-CoA dehydrogenase and acyl-CoA thioesterase II, which catalyze part of the fatty acid beta-oxidation pathway. Indeed, multiple enzymes involved in the fatty acid oxidation pathway were upregulated. Given the presumed structural similarity between alkyl-carboxylic acid side chains and fatty acids, we postulate that P. fluorescens Pf-5 was using existing fatty acid catabolic pathways (among others) during NA degradation. | |
dc.format.extent | 2.18MB | |
dc.format.mimetype | ||
dc.identifier.citation | McKew, B.A., Johnson, R., Clothier, L., Skeels, K., Ross, M.S., Metodiev, M., Frenzel, M., Gieg, L.M., Martin, J.W., Hough, M.A. and Whitby, C. (2021), Differential protein expression during growth on model and commercial mixtures of naphthenic acids in Pseudomonas fluorescens Pf-5. MicrobiologyOpen, 10, article e1196. https://doi.org/10.1002/mbo3.1196 | |
dc.identifier.doi | https://doi.org/10.1002/mbo3.1196 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14078/2551 | |
dc.language | English | |
dc.language.iso | en | |
dc.rights | Attribution (CC BY) | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.subject | naphthenic acids | |
dc.subject | oil sands process-affected water | |
dc.subject | proteomics | |
dc.subject | Pseudomonas fluorescens | |
dc.subject | tailing ponds | |
dc.subject | toxicity | |
dc.title | Differential protein expression during growth on model and commercial mixtures of naphthenic acids in Pseudomonas fluorescens Pf-5 | en |
dc.type | Article |
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