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Flexible molecularly imprinted electrochemical sensor for cortisol monitoring in sweat

Faculty Advisor




biomimetric flexible sensors, capacitive sweat sensors, poly(GMA-co-EGDMA) cortisol imprinted polymer film

Abstract (summary)

A selective cortisol sensor based on molecularly imprinted poly(glycidylmethacrylate-co ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) has been demonstrated for detection of cortisol in human sweat. The non-enzymatic biomimetric flexible sweat sensor was fabricated inexpensively by layer by layer (LbL) assembly. The sensor layers comprised a stretchable polydimethylsiloxane (PDMS) base with carbon nanotubes-cellulose nanocrystals (CNC/CNT) conductive nanoporous nanofilms. The imprinted (MIP) poly(GMA-co-EGDMA) deposited on the CNC/CNT was the cortisol biomimetric receptor. Rapid in analyte response (3 min), the cortisol MIP sensor demonstrated excellent performance. The sensor has a limit of detection (LOD) of 2.0 ng/mL ± 0.4 ng/mL, dynamic range of 10–66 ng/mL, and a sensor reproducibility of 2.6% relative standard deviation (RSD). The MIP sensor also had high cortisol specificity and was inherently blind to selected interfering species including glucose, epinephrine, β-estradiol, and methoxyprogestrone. The MIP was four orders of magnitude more sensitive than its non-imprinted (NIP) counterpart. The MIP sensor remains stable over time, responding proportionately to doses of cortisol in human sweat.

Publication Information

J. Alberkant and S. M. Mugo. Flexible molecularly imprinted electrochemical sensor for cortisol monitoring in sweat. Analytical Bioanalytical Chemistry 2020, 316, 1825-1833. DOI: 10.1007/s00216-020-02430-0


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