Effects of food deprivation and refeeding on neuropeptide Y (NPY) mRNA levels in goldfish

Author
Narnaware, Yuwaraj
Peter, Richard
Faculty Advisor
Date
2001
Keywords
appetite , brain , food intake , food deprivation , growth hormone , gene expression , goldfish , neuropeptide Y , refeeding , starvation
Abstract (summary)
In mammals, NPY is a key factor in the regulation of feeding behavior. In the present study, the effects of refeeding for 1–3 h in 72–75-h food deprived (FD) goldfish on the levels of NPY mRNA in telencephalon-preoptic (TEL-POA), hypothalamus (HYP) and optic tectum-thalamus (OT-THAL) were examined, using Northern blot analysis. Goldfish FD for 72 h exhibited a significant increase in NPY mRNA levels in all brain regions. At 1 h after 72-h FD (73-h FD), NPY mRNA was significantly increased in TEL-POA and OT-THAL, but remained the same as 72-h FD fish in HYP. At 3 h after 72-h FD (75 h), all brain regions exhibited a significant increase in NPY mRNA levels. However, subsequent refeeding for 1–3 h rapidly and completely reversed the effects of FD in all brain regions, reaching fed levels within 1–3 h of refeeding. Serum GH levels were highest in 72-h FD fish, but decreased significantly over 1–3 h after 72-h FD; whereas, refeeding reversed the increase in serum GH levels only at 3 h after refeeding. Taken together, these results further support that NPY is a physiological brain transducer involved in the regulation of daily appetite and feeding in goldfish.
Publication Information
Narnaware, Y.K., & Peter, R.E. (2001a). Effect of food deprivation and refeeding on neuropeptide Y gene expression in goldfish. Comparative Biochemistry and Physiology, 129, 633-637. doi: 10.1016/S1096-4959(01)00359-1
DOI
Notes
Item Type
Article
Language
English
Rights
All Rights Reserved