Effects of food deprivation and refeeding on neuropeptide Y (NPY) mRNA levels in goldfish
Author
Faculty Advisor
Date
2001
Keywords
appetite, brain, food intake, food deprivation, growth hormone, gene expression, goldfish, neuropeptide Y, refeeding, starvation
Abstract (summary)
In mammals, NPY is a key factor in the regulation of feeding behavior. In the present study, the effects of refeeding for 1–3 h in 72–75-h food deprived (FD) goldfish on the levels of NPY mRNA in telencephalon-preoptic (TEL-POA), hypothalamus (HYP) and optic tectum-thalamus (OT-THAL) were examined, using Northern blot analysis. Goldfish FD for 72 h exhibited a significant increase in NPY mRNA levels in all brain regions. At 1 h after 72-h FD (73-h FD), NPY mRNA was significantly increased in TEL-POA and OT-THAL, but remained the same as 72-h FD fish in HYP. At 3 h after 72-h FD (75 h), all brain regions exhibited a significant increase in NPY mRNA levels. However, subsequent refeeding for 1–3 h rapidly and completely reversed the effects of FD in all brain regions, reaching fed levels within 1–3 h of refeeding. Serum GH levels were highest in 72-h FD fish, but decreased significantly over 1–3 h after 72-h FD; whereas, refeeding reversed the increase in serum GH levels only at 3 h after refeeding. Taken together, these results further support that NPY is a physiological brain transducer involved in the regulation of daily appetite and feeding in goldfish.
Publication Information
Narnaware, Y.K., & Peter, R.E. (2001a). Effect of food deprivation and refeeding on neuropeptide Y gene expression in goldfish. Comparative Biochemistry and Physiology, 129, 633-637. doi: 10.1016/S1096-4959(01)00359-1
Notes
Item Type
Article
Language
English
Rights
All Rights Reserved
Permalink
Collections
Loading...