Circular permutated red fluorescent proteins and calcium ion indicators based on mCherry
Red fluorescent indicators for calcium ion (Ca) are preferable, relative to blue-shifted alternatives, for biological imaging applications due to the lower phototoxicity, lower autofluorescent background and deeper tissue penetration associated with longer wavelength light. Accordingly, we undertook the development of a genetically encoded Ca indicator based on the popular and widely utilized Discosoma-derived red fluorescent protein, mCherry.
Carlson, H. J., & Campbell, R. E. (2013). Circular permutated red fluorescent proteins and calcium ion indicators based on mCherry. Protein Engineering, Design & Selection, 26(12), 763–772. https://doi.org/10.1093/protein/gzt052
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