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Heterogeneity of SOX9 and HNF1β in pancreatic ducts is dynamic

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dc.contributor.authorRezanejad, Habib
dc.contributor.authorOuziel-Yahalom, Limor
dc.contributor.authorKeyzer, Charlotte A.
dc.contributor.authorSullivan, Brooke A.
dc.contributor.authorHollister-Lock, Jennifer
dc.contributor.authorLi, Wan-Chun
dc.contributor.authorGuo, Lili
dc.contributor.authorDeng, Shaopeng
dc.contributor.authorLei, Ji
dc.contributor.authorMarkmann, James
dc.contributor.authorBonner-Weir, Susan
dc.date.accessioned2025-04-14T17:20:35Z
dc.date.available2025-04-14T17:20:35Z
dc.date.issued2018
dc.description.abstractPancreatic duct epithelial cells have been suggested as a source of progenitors for pancreatic growth and regeneration. However, genetic lineage-tracing experiments with pancreatic duct-specific Cre expression have given conflicting results. Using immunofluorescence and flow cytometry, we show heterogeneous expression of both HNF1β and SOX9 in adult human and murine ductal epithelium. Their expression was dynamic and diminished significantly after induced replication. Purified pancreatic duct cells formed organoid structures in 3D culture, and heterogeneity of expression of Hnf1β and Sox9 was maintained even after passaging. Using antibodies against a second cell surface molecule CD51 (human) or CD24 (mouse), we could isolate living subpopulations of duct cells enriched for high or low expression of HNF1β and SOX9. Only the CD24high (Hnfβ high/Sox9 high) subpopulation was able to form organoids.
dc.identifier.citationRezanejad, H., Ouziel-Yahalom, L., Keyzer, C. A., Sullivan, B. A., Hollister-Lock, J., Li, W.-C., Guo, L., Deng, S., Lei, J., Markmann, J., & Bonner-Weir, S. (2018). Heterogeneity of SOX9 and HNF1β in pancreatic ducts is dynamic. Stem Cell Reports, 10(3), 725–738. https://doi.org/10.1016/j.stemcr.2018.01.028
dc.identifier.doihttps://doi.org/10.1016/j.stemcr.2018.01.028
dc.identifier.urihttps://hdl.handle.net/20.500.14078/3863
dc.language.isoen
dc.rightsAttribution-NonCommercial-NoDerivs (CC BY-NC-ND)
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectheterogeneity
dc.subjectorganoid
dc.subjectpancreatic ductal cells
dc.titleHeterogeneity of SOX9 and HNF1β in pancreatic ducts is dynamicen
dc.typeArticle

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