Candida antarctica B lipase loaded microreactor for the automated derivatization of lipids

dc.contributor.authorMugo, Samuel
dc.contributor.authorTiedemann, Kyle
dc.date.accessioned2016-10-26
dc.date.accessioned2022-05-28T00:36:24Z
dc.date.available2022-05-28T00:36:24Z
dc.date.issued2016
dc.description.abstractA key bottleneck in the profiling of lipids is the multistep derivatization required prior to gas chromatography (GC) analysis. A single in-vial lipid derivatization and analysis may significantly minimize sample loss and improve analytical sensitivity. A cotton fiber supported poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) polymer microbrush microreactor loaded with Candida antarctica lipase B was developed for the facile conversion of triacylglycerols into fatty acid ethyl ester derivatives for gas chromatograph – mass spectrometry (GC-MS) analysis. The polymer microbrush microreactor was fabricated in effort to provide efficient, simplified, cost-effective, and high throughput GC-MS determination of triacylglycerols. The polymer microbrush microreactor was used as an in-vial triacylglycerol transesterification platform, with economical sample consumption of less than or equal to 100 µL and significant reduction of reagents. To evaluate the polymer microbrush microreactor performance for lipids, a triolein standard and camelina oil triacylglycerols were quantitatively transformed into ethyl oleate and fatty acid ethyl esters, respectively, following a three hour reaction time. The lipase loaded cotton fiber supported poly (glycidylmethacrylate-co-ethylene glycol dimethacrylate) polymer microbrush microreactors were reusable for up to five times for quantitative transesterification with minimal loss of lipase activity.
dc.description.urihttps://library.macewan.ca/cgi-bin/SFX/url.pl/8N2
dc.identifierhttps://doi.org/10.1080/00032719.2016.12
dc.identifier.citationMugo, S. M., & Tiedemann, K. (2016). Candida antarctica B lipase loaded microreactor for the automated derivatization of lipids. Analytical Letters (accepted). doi:10.1080/00032719.2016.12
dc.identifier.urihttps://hdl.handle.net/20.500.14078/618
dc.languageEnglish
dc.language.isoen
dc.rightsAll Rights Reserved
dc.subjectgas chromatography
dc.subjectlipases
dc.subjectpolymer microbrush Microreactor
dc.subjecttransesterification
dc.titleCandida antarctica B lipase loaded microreactor for the automated derivatization of lipids
dc.typeArticle
dspace.entity.type
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