Candida antarctica B lipase loaded microreactor for the automated derivatization of lipids
dc.contributor.author | Mugo, Samuel | |
dc.contributor.author | Tiedemann, Kyle | |
dc.date.accessioned | 2016-10-26 | |
dc.date.accessioned | 2022-05-28T00:36:24Z | |
dc.date.available | 2022-05-28T00:36:24Z | |
dc.date.issued | 2016 | |
dc.description.abstract | A key bottleneck in the profiling of lipids is the multistep derivatization required prior to gas chromatography (GC) analysis. A single in-vial lipid derivatization and analysis may significantly minimize sample loss and improve analytical sensitivity. A cotton fiber supported poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) polymer microbrush microreactor loaded with Candida antarctica lipase B was developed for the facile conversion of triacylglycerols into fatty acid ethyl ester derivatives for gas chromatograph – mass spectrometry (GC-MS) analysis. The polymer microbrush microreactor was fabricated in effort to provide efficient, simplified, cost-effective, and high throughput GC-MS determination of triacylglycerols. The polymer microbrush microreactor was used as an in-vial triacylglycerol transesterification platform, with economical sample consumption of less than or equal to 100 µL and significant reduction of reagents. To evaluate the polymer microbrush microreactor performance for lipids, a triolein standard and camelina oil triacylglycerols were quantitatively transformed into ethyl oleate and fatty acid ethyl esters, respectively, following a three hour reaction time. The lipase loaded cotton fiber supported poly (glycidylmethacrylate-co-ethylene glycol dimethacrylate) polymer microbrush microreactors were reusable for up to five times for quantitative transesterification with minimal loss of lipase activity. | |
dc.description.uri | https://library.macewan.ca/cgi-bin/SFX/url.pl/8N2 | |
dc.identifier.citation | Mugo, S. M., & Tiedemann, K. (2016). Candida antarctica B lipase loaded microreactor for the automated derivatization of lipids. Analytical Letters (accepted). doi:10.1080/00032719.2016.12 | |
dc.identifier.doi | https://doi.org/10.1080/00032719.2016.12 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14078/618 | |
dc.language | English | |
dc.language.iso | en | |
dc.rights | All Rights Reserved | |
dc.subject | gas chromatography | |
dc.subject | lipases | |
dc.subject | polymer microbrush Microreactor | |
dc.subject | transesterification | |
dc.title | Candida antarctica B lipase loaded microreactor for the automated derivatization of lipids | en |
dc.type | Article |