Reinforcing one-carbon metabolism via folic acid/Folr1 promotes β-cell differentiation
| dc.contributor.author | Karampelias, Christos | |
| dc.contributor.author | Rezanejad, Habib | |
| dc.contributor.author | Rosko, Mandy | |
| dc.contributor.author | Duan, Likun | |
| dc.contributor.author | Lu, Jing | |
| dc.contributor.author | Pazzagli, Laura | |
| dc.contributor.author | Bertolino, Philippe | |
| dc.contributor.author | Cesta, Carolyn E. | |
| dc.contributor.author | Liu, Xiaojing | |
| dc.contributor.author | Korbutt, Gregory S. | |
| dc.contributor.author | Andersson, Olov | |
| dc.date.accessioned | 2025-04-14T17:01:05Z | |
| dc.date.available | 2025-04-14T17:01:05Z | |
| dc.date.issued | 2021 | |
| dc.description.abstract | Diabetes can be caused by an insufficiency in β-cell mass. Here, we performed a genetic screen in a zebrafish model of β-cell loss to identify pathways promoting β-cell regeneration. We found that both folate receptor 1 (folr1) overexpression and treatment with folinic acid, stimulated β-cell differentiation in zebrafish. Treatment with folinic acid also stimulated β-cell differentiation in cultures of neonatal pig islets, showing that the effect could be translated to a mammalian system. In both zebrafish and neonatal pig islets, the increased β-cell differentiation originated from ductal cells. Mechanistically, comparative metabolomic analysis of zebrafish with/without β-cell ablation and with/without folinic acid treatment indicated β-cell regeneration could be attributed to changes in the pyrimidine, carnitine, and serine pathways. Overall, our results suggest evolutionarily conserved and previously unknown roles for folic acid and one-carbon metabolism in the generation of β-cells. | |
| dc.identifier.citation | Karampelias, C., Rezanejad, H., Rosko, M., Duan, L., Lu, J., Pazzagli, L., Bertolino, P., Cesta, C. E., Liu, X., Korbutt, G. S., & Andersson, O. (2021). Reinforcing one-carbon metabolism via folic acid/Folr1 promotes β-cell differentiation. Nature Communications, 12(1), 3362–13. https://doi.org/10.1038/s41467-021-23673-0 | |
| dc.identifier.doi | https://doi.org/10.1038/s41467-021-23673-0 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.14078/3862 | |
| dc.language.iso | en | |
| dc.rights | Attribution (CC BY) | |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | regeneration | |
| dc.subject | zebrafish | |
| dc.subject | diabetes | |
| dc.title | Reinforcing one-carbon metabolism via folic acid/Folr1 promotes β-cell differentiation | en |
| dc.type | Article |
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