Effects of super-class cannabis terpenes beta-caryophyllene and alpha-pinene on zebrafish behavioural biomarkers
| dc.contributor.author | Johnson, Andrea | |
| dc.contributor.author | Stewart, Alycia | |
| dc.contributor.author | El-Hakim, Ismaeel | |
| dc.contributor.author | Hamilton, Trevor | |
| dc.date.accessioned | 2023-06-15T19:19:04Z | |
| dc.date.available | 2023-06-15T19:19:04Z | |
| dc.date.issued | 2022 | |
| dc.description.abstract | Terpenes possess a wide range of medicinal properties and are potential therapeutics for a variety of pathological conditions. This study investigated the acute effects of two cannabis terpenes, β-caryophyllene and α-pinene, on zebrafish locomotion, anxiety-like, and boldness behaviour using the open field exploration and novel object approach tests. β-caryophyllene was administered in 0.02%, 0.2%, 2.0%, and 4% doses. α-pinene was administered in 0.01%, 0.02%, and 0.1% doses. As α-pinene is a racemic compound, we also tested its (+) and (−) enantiomers to observe any differential effects. β-caryophyllene had only a sedative effect at the highest dose tested. α-pinene had differing dose-dependent effects on anxiety-like and motor variables. Specifically, (+)-α-pinene and (−)-α-pinene had significant effects on anxiety measures, time spent in the thigmotaxis (outer) or center zone, in the open field test, as well as locomotor variables, swimming velocity and immobility. (+ /−)-α-pinene showed only a small effect on the open field test on immobility at the 0.1% dose. This study demonstrates that α-pinene can have a sedative or anxiolytic effect in zebrafish and may have different medicinal properties when isolated into its (+) or (−) enantiomers. | |
| dc.identifier.citation | Johnson, A., Stewart, A., El-Hakim, I. et al. Effects of super-class cannabis terpenes beta-caryophyllene and alpha-pinene on zebrafish behavioural biomarkers. Sci Rep 12, 17250 (2022). https://doi.org/10.1038/s41598-022-21552-2 | |
| dc.identifier.doi | https://doi.org/10.1038/s41598-022-21552-2 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.14078/3135 | |
| dc.language.iso | en | |
| dc.rights | Attribution (CC BY) | |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | terpenes | |
| dc.subject | zebrafish | |
| dc.subject | behavioral neuroscience | |
| dc.subject | open field test | |
| dc.title | Effects of super-class cannabis terpenes beta-caryophyllene and alpha-pinene on zebrafish behavioural biomarkers | en |
| dc.type | Article |
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