Repository logo
 

9-(2-Phosphonyl-methoxyethyl)-adenine promotes erythrocytic differentiation and disrupts cell replication in chronic myelogenous leukemia K562 cells

Simple item page
dc.contributor.authorWiseman, Brittany
dc.date.accessioned2022-03-11
dc.date.accessioned2022-05-31T01:50:52Z
dc.date.available2022-05-31T01:50:52Z
dc.date.issued2021
dc.description.abstractDisruption during cellular differentiation can cause hematopoietic stem cells to proliferate uncontrollably, resulting in the development of cancer. Differentiation therapies are being investigated as a type of cancer treatment which involve inducing agents that promote the differentiation of cancer cells into those with similar properties to normal blood cells. These cells can then undergo apoptosis at an accelerated and controlled rate compared to cancer cells, making this a potential therapeutic technique. In this study, the ability of human chronic myelogenous leukemia K562 cells to undergo cellular differentiation in response to the inducing agent 9-(2-Phosphonyl-methoxy ethyl)-adenine (PMEA) is investigated. PMEA has previously been shown to disrupt cell replication, and promote erythrocytic differentiation in K562 cells. In order to further test the effectiveness of this inducer, cell proliferation was measured with a cell growth curve, hemoglobin presence was measured with benzidine staining, and gamma-globin expression (a protein subunit of fetal hemoglobin) was measured in both induced and uninduced K562 cell cultures via RT-qPCR and western blotting. The results indicate that PMEA slows cell replication, and promotes hemoglobin (and subsequently gamma-globin) expression in treated cells. In summary, the findings support the conclusion that PMEA is able to promote erythrocytic differentiation in K562 cells, and provides information that supports differentiation therapies as a method for cancer treatment.
dc.format.extent647.92KB
dc.format.mimetypePDF
dc.identifier.citationWiseman, B. (2021). 9-(2-Phosphonyl-methoxyethyl)-adenine promotes erythrocytic differentiation and disrupts cell replication in chronic myelogenous leukemia K562 cells. MacEwan University Student EJournal (MUSe), 5(1). https://doi.org/10.31542/muse.v5i1.2000
dc.identifier.doihttps://doi.org/10.31542/muse.v5i1.2000
dc.identifier.urihttps://hdl.handle.net/20.500.14078/2613
dc.languageEnglish
dc.language.isoen
dc.rightsAttribution-NonCommercial (CC BY-NC)
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.subjectK562
dc.subjectcellular differentiation
dc.subjectPMEA
dc.subjectgamma globin
dc.subjectdifferentiation therapy
dc.title9-(2-Phosphonyl-methoxyethyl)-adenine promotes erythrocytic differentiation and disrupts cell replication in chronic myelogenous leukemia K562 cellsen
dc.typeStudent Article

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
9-(2-Phosphonyl-methoxyethyl)-adenine_promotes-_2021_roam.pdf
Size:
647.92 KB
Format:
Adobe Portable Document Format
Download

Collections

Biological Sciences - Student Works